Determination of Volatile Components from Flowers of 7 Species of Cerasus in Zhejiang Province by HS-SPME-GC-MS
Ningbo Key Laboratory of Landscape Plant Development / Ningbo City College of Vocational Technology, Ningbo, 315100, China
Bioscience Methods, 2021, Vol. 12, No. 3 doi: 10.5376/bm.2021.12.0003
Received: 14 Oct., 2020 Accepted: 10 Feb., 2021 Published: 20 Feb., 2021
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This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Fu T., Wang Z.L., Li W., and Chen J.S., 2021, Determination of volatile components from flowers of 7 species of cerasus in Zhejiang Province by HS-SPME-GC-MS, Bioscience Method, 12(3): 1-8 (doi: 10.5376/bm.2021.12.0003)
To provide a theoretical basis for utilizing the resources of Cerasus plants in China, the volatile components among 7 species of Cerasus in Zhejiang Province were compared to understand genetic relationship. The samples were treated by HS-SPME and the pretreatment conditions were optimized. Combined with GC-MS technology, the volatile components from flowers of 7 species of Cerasus were determined, and the difference of them was compared. Then, SPSS 22 software was applied to conduct the PCA of the 7 species of Cerasus flowers. According to the score diagrams, the relationship among the 7 species of Cerasus was visually presented. By optimizing treatment conditions of the samples, the best extraction conditions of SPME were 1.0 g of sample weight, 70℃ of heating temperature, 40 min of adsorption. By GC-MS qualitative analysis，42 volatile components were identified from the 7 species of Cerasus, among them, there are 21 kinds of substances identified by Cerasus subhirtella var. ascendens and 7 kinds by Cerasus jingningensis, it is mainly aldehydes, alcohols and esters, with less organic acids, ketones and hydrocarbons, among which aldehydes account for more than 88%, and benzaldehyde is the highest among aldehydes, with the content of more than 80%, this component is not only high in content, but also low in aroma threshold, which plays an important role in the special smell of Cerasus plants. The results of PCA showed that the other Cerasus plants gathered together, except for the macrophytes, which supported the morphological classification to classify them into the Subgen. Micro Cerasus. The results of this study can provide a theoretical basis for the development and utilization of Cerasus resources in China.
Cerasus; Gas chromatography-mass spectrometry (GC-MS); Volatile components; PCA analysis