Research Article

Cloning and Bioinformatics Analysis of the EfNAC Gene in Erianthus fulvus  

Shujie Gu* , Xianyue Shen* , Zenfeng Qian , Dan Zeng , Hao Ma , Yining Di , Lilian He , Fusheng Li
College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201
* These authors contributed equally to this work
Author    Correspondence author
Computational Molecular Biology, 2021, Vol. 11, No. 3   doi: 10.5376/cmb.2021.11.0003
Received: 01 Sep., 2020    Accepted: 01 Dec., 2020    Published: 19 Mar., 2021
© 2021 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Gu S.J., Shen X.Y., Qian Z.F., Zeng D., Ma H., Di Y.N., He L.L., and Li F.S., 2021, Cloning and bioinformatics analysis of the EfNAC gene in Erianthus fulvus, Computational Molecular Biology, 11(3): 1-10 (doi: 10.5376/cmb.2021.11.0003)

Abstract

In order to make better use of the resistance-related genes of sugarcane wild species Erianthus fulvus Ness., and to provide reliable candidate genes for resistance breeding of transgenic sugarcane. In this research, we used electronic cloning and RT-PCR to clone a gene that was induced by drought stress in the wild species of Erianthus fulvus (Kun ming 99-2), and named it FfNAC (GenBank ID: MT499790). Bioinformatics analysis shows that the ORF of the EfNAC is 765 bp in length, encodes 254 amino acids in total, and has a conserved NAM Superfamily. EfNAC protein is mainly composed of random coils and alpha helices, without signal peptide, with multiple phosphorylation sites, and no transmembrane structure. this research provides theoretical basis and technical support for in-depth research of the molecular regulation mechanism of drought stress in the sugarcane and the drought-resistant breeding of transgenic sugarcane.

Keywords
Sugarcane; Erianthus fulvus; EfNAC gene; Drought resistance
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