Establishment and Identification of Cardiomyocyte-specific High Expression Human APE1 Transgenic Mouse
1 Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Clinnical Research Center, The Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001
2 Maternal and Child Research Institute, Shunde Women and Children’s Hospital of Guangdong Medical University, Maternal and Child Health Hospital of Shunde District, Foshan, Guangdong, 510080
Genomics and Applied Biology, 2019, Vol. 10, No. 2 doi: 10.5376/gab.2019.10.0002
Received: 27 Sep., 2019 Accepted: 20 Nov., 2019 Published: 29 Oct., 2019
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Preferred citation for this article:
iang J.M., Wang P., Li S.H., Wang Z.Q., Wu K., Guo R.M., 2019, Establishment and identification of cardiomyocyte-specific high expression human APE1 transgenic mouse, Genomics and Applied Biology, 10(2): 10-15 (doi: 10.5376/gab.2019.10.0002)
The aim of this study is to establish transgenic mice that specifically over-express human APE1 incardiac, and to provide a tool animal for studying the relationship between the function and mutation of hAPE1 gene and cardiac development and cardiovascular diseases. A cardiomyocyte-specific hAPE1 transgenic construct containing the α-myosin heavy chain (α-MHC) promoter and human A PE1 (hA PE1) gene was generated. The transgenic vector was constructed by insertion of hAPE1 gene under the α-MHC promoter. The transgenic mice were generated by fertilized egg microinjection followed by embryo transplantation and were all maintained on C57BL/6J genetic background. The genotype of transgenic mice was identified using PCR and the expression levels of hAPE1 in different tissues were detected by Western blotting. The results indicated that cardiomyocyte-specific hAPE1 transgenic construct containing the α-myosin heavy chain (α-MHC) promoter and human APE1 (hAPE1) gene were introduced into fertilized zygotes by microinjection, and then the fertilized zygotes were implanted into the oviduct of female mice, establishing a heart specific high expression hAPE1 transgenic mouse line. 40 offspring were obtained and 15 mice carrying the human APE1 gene was identified by PCR. The heart-specific overexpression of hAPE1 was confirmed by Western blotting assay. The present study successfully obtained cardiomyocyte-specific hAPE1 transgenic mice transgenic mice expressing, which provided a useful tool for studying the function of genes in heart development and cardiovascular disease.
APE1; Transgenic; Heart; Mouse; Tissue specific
Genomics and Applied Biology
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