Analysis of resistance to alkaline salt stress in Arabidopsis thaliana with overexpression of OsiSAP1 gene
Key Laboratory of Saline and Alkali vegetation Rehabilitation and Reconstruction in Northeast China ，School of Life Science ，Northeast Forestry University，Harbin 150040，China
Genomics and Applied Biology, 2020, Vol. 11, No. 2
Received: 21 Apr., 2020 Accepted: 23 Apr., 2020 Published: 26 Apr., 2020
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The purpose of this study was to study the stress effects of rice ISAP transcription factor family containing zinc finger proteins of A20/AN1 domain and the mechanism of saline-alkali tolerance during rice growth and development. In this study, the ORF reading frame of OsiSAP1 (LOC_Os09g31200) gene 513bp was cloned from the leaves of rice Oryza sativa. L11, and the homology ratio NCBI 170 amino acids was as high as 71.51% with Panicum miliaceum L. The phylogenetic tree based on the nucleotide comparison of the open reading frame shows that it is on the same branch as Oryza officinalis. Through qRT-PCR detection in various tissues and organs of rice Oryza sativa. L11: root, stem, leaf and anther, it was found that the expression of mRNA in root was higher, and Gene gun-transformed onion epidermal cell method detected fluorescence signal of 35S-OsiSAP1-GFP fusion protein localized in the nucleus. The analysis of salt-alkali tolerance of rice overexpressing OsiSAP1 gene showed that the fresh weight and root length of transgenic lines were better than those of wild-type lines at germination stage and late germination stage under NaHCO3 treatment. It is speculated that OsiSAP1 transcription factors are involved in the regulation of saline-alkali tolerance in rice.
Arabidopsis thaliana; Stress protein; Alkaline salt stress; Expression characteristics
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