Enhanced biosynthesis of lomofungin via overexpressing S-adenosylmethionine synthetase and optimizing carbon source
State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China
Genomics and Applied Biology, 2020, Vol. 11, No. 3
Received: 06 May, 2020 Accepted: 07 May, 2020 Published: 07 May, 2020
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This article was first published in 《基因组学与应用生物学》(ISSN1674-568X，CN45-1369/Q) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Lomofungin, generally biosynthesized by Streptomyces lomondensis S015, is a kind of phenazine compound which has broad-spectrum antifungal, antibacterial, and antitumor activities. The low-level production of lomofungin in S015 wild-type strain has been a limitation for its widely application. In order to enhance the production of lomofungin, investigate the function of the related gene, and explore the fermentation condition of S015 strain, phylogenetic tree construction was carried out for analysis of S-adenosylmethionine synthetase MetK firstly. Second, metK gene was overexpressed in wild-type S015. Carbon source of culture medium was optimized by time-course fermentation at last. The production of lomofungin reached 27.4 ± 2.8 mg/L by overexpressing metK gene, about 2.3-fold higher than that in wild-type S015. Carbon source optimization showed that a highest lomofungin production (130.6 ± 8.0 mg/L) was obtained while using 20g/L xylose, increasing about 4.8-fold compared with the unoptimized condition. This study took advantage of up-stream gene manipulation and down-stream fermentation optimization strategy, significantly enhanced the production of lomofungin.
Lomofungin; S-adenosylmethionine synthetase gene metK; Gene overexpression; Fermentation optimization
Genomics and Applied Biology
• Volume 11