The expression of miR-99b in the differentiation of bovine muscle satellite cells and prediction of target genes
Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, College of Life Sciences, Agriculture and Forestry, Qiqihar University, Qiqihar, 161006
Genomics and Applied Biology, 2020, Vol. 11, No. 4
Received: 10 Jul., 2020 Accepted: 10 Jul., 2020 Published: 10 Jul., 2020
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This article was first published in 《基因组学与应用生物学》(ISSN1674-568X，CN45-1369/Q) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
As an evolutionarily conserved non-coding RNA, miRNA plays an important role in the occurrence, development and proliferation of cells. miR-99b is involved in the differentiation and proliferation of various cells, but its expression in bovine muscle satellite cells and its possible target genes are unclear. This study first introduced the location and sequence information of miR-99b in the bovine genome. The stem-loop fluorescence quantitative PCR technique was used to detect the expression of miR-99b under the proliferation and differentiation of bovine muscle satellite cells. The results showed that the expression of the proliferation state was higher than the expression of different periods of differentiation. The miRNA binds the complementary sequence on the messenger RNA transcript, which can simultaneously regulate the expression of multiple target genes after transcription. In order to further study the regulatory role and mechanism of miR-99b, this study used online software to predict its possible target genes. It was found that about 58 gene in cattle, the 3'UTR of mRNA contains miR-99b potential binding sites, and most of the target genes are related to cell proliferation, differentiation, and apoptosis. This study provides a theoretical basis and experimental basis for detecting the mechanism of action of miR-99b in bovine skeletal muscle satellite cells.
miR-99b; Bovine, Differentiation; Target gene prediction
Genomics and Applied Biology
• Volume 11