Research Report

Expression of a Seed Storage Protein AtSSA3 of Arabidopsis thaliana in Escherichia coli  

Le Chang1,2 , Shenkui Liu3 , Yuanyuan Bu1,2
1 Key Laboratory of Saline-Alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, Harbin 150040, China
2 College of Life Science, Northeast Forestry University, Harbin 150040, China
3 The State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin’An, 311300, China
Author    Correspondence author
Molecular Soil Biology, 2021, Vol. 12, No. 1   doi: 10.5376/msb.2021.12.0001
Received: 02 Mar., 2021    Accepted: 03 Mar., 2021    Published: 10 Mar., 2021
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Chang L., Liu S.K., and Bu Y.Y., 2021, Expression of a seed storage protein AtSSA3 of Arabidopsis thaliana in Escherichia coli, Molecular Soil Biology, 12(1): 1-6 (doi:10.5376/msb.2021.12.0001)

Abstract

Mature seeds are rich in storage proteins, which not only affect the quality of seeds, but also the energy source of seed germination and seedling growth. In this study, a gene encoding a seed storage protein SSA3 from Arabidopsis thaliana was isolated. The AtSSA3 gene was expressed in Escherichia coli BL21 (DE3). The fusion protein pET-32a(+)-AtSSA3 was induced under the optimized conditions of 1mM IPTG and 25°C for 4h. Western blot analysis the optimal conditions of protein expression. This study provides a theoretical and practical basis for the further study of seed storage protein genes in the future.

Keywords
AtSSA3; Protein induction; Prokaryotic expression
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