Expression of a Seed Storage Protein AtSSA3 of Arabidopsis thaliana in Escherichia coli
1 Key Laboratory of Saline-Alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, Harbin 150040, China
2 College of Life Science, Northeast Forestry University, Harbin 150040, China
3 The State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin’An, 311300, China
Molecular Soil Biology, 2021, Vol. 12, No. 1 doi: 10.5376/msb.2021.12.0001
Received: 02 Mar., 2021 Accepted: 03 Mar., 2021 Published: 10 Mar., 2021
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Preferred citation for this article:
Chang L., Liu S.K., and Bu Y.Y., 2021, Expression of a seed storage protein AtSSA3 of Arabidopsis thaliana in Escherichia coli, Molecular Soil Biology, 12(1): 1-6 (doi:10.5376/msb.2021.12.0001)
Mature seeds are rich in storage proteins, which not only affect the quality of seeds, but also the energy source of seed germination and seedling growth. In this study, a gene encoding a seed storage protein SSA3 from Arabidopsis thaliana was isolated. The AtSSA3 gene was expressed in Escherichia coli BL21 (DE3). The fusion protein pET-32a(+)-AtSSA3 was induced under the optimized conditions of 1mM IPTG and 25°C for 4h. Western blot analysis the optimal conditions of protein expression. This study provides a theoretical and practical basis for the further study of seed storage protein genes in the future.
AtSSA3; Protein induction; Prokaryotic expression