Research Report

Analysis of ATH1 Transcription Activity and Co-IP of ATH1 Protein Interaction with OFP1  

Liguo Zhang1,2 , Xiaofei Zhang1 , Guijiang Wang1 , Shuquan Zhang1 , Ying Chang2
1 Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, China
2 Northeast Agricultural University, Harbin, 150038, China
Author    Correspondence author
Biological Evidence, 2023, Vol. 13, No. 1   doi: 10.5376/be.2023.13.0001
Received: 22 Jul., 2023    Accepted: 31 Jul., 2023    Published: 03 Aug., 2023
© 2023 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Zhang L.G., Zhang X.F., Wang G.J., Zhang S.Q., and Chang Y., 2023, Analysis of ATH1 transcription activity and Co-IP of ATH1 protein interaction with OFP1, Bioscience Evidence, 13(1): 1-4 (doi: 10.5376/be.2023.13.0001)


ATH1 has multifaceted functions in regulating growth and development, and possibly regulate development by interacting with OFP1 protein. However, the protein interaction of ATH1 with OFP1 has not been further confirmed, and the transcription activity of ATH1 has not been determined. In this work, the interaction ATH1 with OFP1 was further confirmed by using Co-IP, and the transcription activity of ATH1 was determined by using Protoplast transfection system. The results showed that the ATH1-OFP1 protein complexes could be formed in vivo, and ATH1 was a transcription inhibitory factor, and OFP1 could enhance the transcription inhibitory activity of ATH1. This study will provide molecular basis for analysis of the regulatory functions of OFP-TALE complexes in plant development, which is great significance for improving important plant traits.

ATH1; Transcriptional activity; Protein interaction; Co-IP
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