Li M.M., 2024, Process study on microbial fixation of CO₂ and its conversion into organic acids, Bioscience Evidence, 14(4): 143-153 (doi: 10.5376/be.2024.14.0016)

The study identified several natural and synthetic CO₂ fixation pathways, including the Calvin cycle, the Wood-Ljungdahl pathway, and the 3-hydroxypropionate cycle, among others. Key enzymes such as ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and formate dehydrogenase were found to play crucial roles in these pathways. The research also highlighted the potential of specific bacterial strains, such as Bacillus sp. SS105, in enhancing CO₂ sequestration and lipid production for biodiesel applications. Additionally, the study demonstrated that metabolic engineering and optimization of microbial consortia could significantly improve the yields of organic acids like succinic acid and butyric acid. The findings of this study underscore the potential of microbial CO₂ fixation as a viable strategy for reducing greenhouse gas emissions and producing valuable organic acids. The identification of efficient microbial pathways and key enzymes, along with advancements in metabolic engineering, paves the way for future applications in sustainable chemical production and biofuel generation. Further research should focus on optimizing these processes to enhance their industrial applicability and economic feasibility.