Wei  Wang , Jia  Xuan

Biotechnology Research Center, Cuixi Academy of Biotechnology, Zhuji, 311800, China
Author    Correspondence author
Biological Evidence, 2025, Vol. 15, No. 2   
Received: 01 Mar., 2025    Accepted: 03 Apr., 2025    Published: 11 Apr., 2025

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cordyceps polysaccharides have become a research focus in the field of functional foods and pharmaceuticals due to their strong biological activity and wide application prospects. Supercritical fluid technology, especially supercritical carbon dioxide (SC-CO₂) technology, has the characteristics of high efficiency, environmental protection, and strong selectivity. This study systematically summarized the extraction methods of Cordyceps polysaccharides, with a focus on the application status and process optimization of supercritical fluid technology in Cordyceps polysaccharide extraction. The key factors affecting extraction efficiency were analyzed, and the application of response surface methodology (RSM) in optimizing extraction processes was explored, as well as its role in improving polysaccharide yield and purity. The key technologies for polysaccharide quality control were also summarized. In response to the potential of supercritical fluid technology in the industrialization of Cordyceps polysaccharide extraction, this study proposes directions for green process improvement, multi-objective extraction strategies, and equipment upgrades, providing scientific basis for efficient extraction and quality control of Cordyceps polysaccharides.

Cordyceps polysaccharide; Supercritical fluid technology; Extraction efficiency; Process optimization; Biological activity; Green technology