Fei Zhao , Fan Wang

Aquatic Biology Research Center, Cuixi Academy of Biotechnology, Zhuji, 311800, Zhejiang, China
Author    Correspondence author
Genomics and Applied Biology, 2024, Vol. 15, No. 2   doi: 10.5376/gab.2024.15.0011
Received: 17 Feb., 2024    Accepted: 21 Mar., 2024    Published: 03 Apr., 2024

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Zhao F., and Wang F., 2024, Efficient gene transfer techniques in shrimp and their cellular applications, Genomics and Applied Biology, 15(2): 89-98 (doi: 10.5376/gab.2024.15.0011)

This study compared the efficiency of microinjection, electroporation, and transfection methods for gene transfer into shrimp zygotes. Transfection using the jetPEI reagent demonstrated the highest efficiency, with hatching rates of 50%-60% and gene expression rates of 40%-60%. Additionally, a VP28-pseudotyped baculovirus system achieved up to 100% infection efficiency in adult shrimp tissues, although it exhibited tissue-specific tropism. A triple-pseudotyped retroviral system also showed promise, particularly in shrimp primary lymphoid cells, with infection efficiencies of 20%-30%. Furthermore, the inclusion of shrimp-specific promoters and viral envelope proteins significantly enhanced the tropism and infectivity of lentiviral vectors in shrimp cells. The findings indicate that transfection with jetPEI and the use of pseudotyped viral systems are highly effective for gene transfer in shrimp. These methods hold significant potential for advancing genetic manipulation and cellular studies in shrimp, which could lead to improved disease resistance and other desirable traits in aquaculture.

Gene transfer; Shrimp; Transfection; Electroporation; Microinjection; Pseudotyped viral systems; jetPEI; VP28; Lentiviral vectors; Aquaculture