Xiaoyuan Chi , Qingli Yang , Lijuan Pan , Yanan He , Mingna Chen , Yuan Gao , Shanlin Yu

Shandong Peanut Research Institute, Qingdao, 266100, P.R. China
Author    Correspondence author
Genomics and Applied Biology, 2010, Vol. 1, No. 2   doi: 10.5376/gab.2010.01.0002
Received: 15 Aug., 2010    Accepted: 19 Sep., 2010    Published: 28 Sep., 2010

This is an Open Access article published under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Chi et al., 2010, Cloning and Expression Analysis of β-ketoacyl-ACP Reductase, β-hydroxyacyl-ACP Dehydrase and enoyl-ACP Reductase from Arachis hypogaea L., Genomics and Applied Biology, 2010, Vol. 1, No. 2 (doi: 10.5376/gab.2010.01.0002)

Fatty acid biosynthesis is catalysed in most bacteria and plants by a group of highly conserved proteins known as the Type II fatty acid synthase (FAS) system. In this study, genes for β-ketoacyl-ACP reductase (KR), β-hydroxyacyl-ACP dehydrase (HD), and enoyl-ACP reductase (ENR) of Type II FAS have been cloned from peanut (Arachis hypogaea L.). The results showed that the ORF of the three genes were 972 bp, 651 bp and 1170 bp in length, encoding 323, 216 and 389 amino acids, respectively. The predicted amino acid sequences of AhKR, AhHD, AhENR shared high sequence identity of 86.3%, 81.2% and 87.2% to the corresponding ones in Glycine max, respectively. Further investigation of quantitative real-time RT-PCR analysis suggested that AhKR, AhHD and AhENR were expressed with higher levels in leaf and seed than those in root and stem tissues. Moreover, AhKR and AhENR genes reached a maximum expression level at 25 DAP (days after pegging) and showed a downward trend thereafter. In contrast, AhHD gene expressed in an irregular course during seed development. Overall, the information generated by this study will facilitate the manipulation of the quality of oils produced in seeds of oil crops.

Peanut (Arachis hypogaea L.); Fatty acid biosynthesis; β-ketoacyl-ACP reductase (KR); β-hydroxyacyl-ACP dehydrase (HD); Enoyl-ACP reductase (ENR); Expression analysis