International joint research led by Akihisa Osakabe and Yoshimasa Takizawa of the University of Tokyo has clarified the molecular mechanisms in thale cresses (Arabidopsis thaliana) by which the DDM1 (Decreased in DNA Methylation 1) protein prevents the transcription of "jumping genes." DDM1 makes "jumping genes" more accessible for transcription-suppressing chemical marks to be deposited. Because a variant of this protein exists in humans, the discovery provides insight into genetic conditions caused by such "jumping gene" mutations.

 

Disentangled DNA is often referred to as a "string." In a cell, however, it looks more like a "string ball," only the looping patterns are much more complex. The smallest unit is called a nucleosome. It consists of a section of DNA wrapped around a protein (histone) scaffolding. Transposons, genes that can "jump" to different locations in the genome, are "tucked away" in nucleosomes, which makes it difficult for the cell to deposit chemical marks that suppress transposon transcription. DDM1 is a protein known for maintaining such suppressing chemical marks, but it has not been clear how it can access transposons when they are neatly "tucked away." The researchers used cryo-electron microscopy, a technique capable of imaging at near-atomic scales. This allowed them to look at the structure of the DDM1 protein and DNA within the nucleosome.

 

The high-resolution images showed the exact positions where DDM1 bound to the DNA in the nucleosome.As a result, the specific binding site, which normally closes the nucleosome, got more "flexible" and opened up to allow suppressing chemical marks to be deposited, preventing transposons from being transcribed. This seemingly minor detail could be the start of major improvements. In the long term, such discoveries could lead to new treatments for genetic diseases in humans caused by similar genes. This new knowledge also provides insights into how plants and other organisms control their DNA, which could improve our ability to grow better crops or develop new biotechnologies.