Sheengh Yu

Institute of Life Sciences, Jiyang Colloge of Zhejiang A&F University, Zhuji, 311800, Zhejiang, China
Author    Correspondence author
Biological Evidence, 2025, Vol. 15, No. 2   
Received: 22 Feb., 2025    Accepted: 23 Mar., 2025    Published: 31 Mar., 2025

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

In recent years, some research achievements have been made in using molecular breeding methods to improve the oleic acid content of rapeseed, with a focus on introducing several commonly used new technologies, such as gene editing, marker assisted selection (MAS), and gene regulation. Special mention was made of gene editing tools such as CRISPR/Cas9, which can directly modify key genes like FAD2. There are also some studies on transcription factors that have discovered how these genes control oleic acid levels. Through QTL mapping technology, scientists have also identified genetic loci related to oleic acid content. This study also analyzed how traditional breeding and modern molecular breeding can be combined, discussed some existing problems in current research, such as the impact of environmental factors on breeding effectiveness, and proposed that these challenges can be solved in the future through multi omics data integration, improving adaptability, and other methods.

Oleic acid; Rapeseed breeding; CRISPR/Cas9; Marker-assisted selection; Fatty acid biosynthesis