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Isolation and Resistance Analysis of Algae of Alkali Spot Soil

Algae can survive in a very wide range environment and have high adaptability for a variety of adversity. In present study, we selected alkali spot soil in Anda City, Heilongjiang Province as the algae research plots. In addition, we selected garden nursery soil in Northeast Forestry University a... (28 Jun., 2011)

Studies on Construction of Regeneration System and Genetic Transformation of Puccinellia chinampoensis

The regeneration system was established and the genetic transformation was processed, using the mature seeds of Puccinellia chinampoensis as explants. The results showed that the callus induction rate could reach to 39.75% after 19days of induction with the altered mediumâ… (adding 4 mg/L 2,4-... (23 Jun., 2011)

Isolation, Biochemical Characterization and Anti-adhesion Property of Mucin from the Blue Blubber Jellyfish (Catostylus mosaicus)

Mucins are large glycoproteins that have been identified as the main component of various biological gels and lubricants. Their compositions contribute to the viscoelastic properties of mucus secretions. Gel-forming mucins have now been identified in a variety of organisms from marine molluscs to... (03 Jun., 2011)

Quantitative Determination for Nerve Growth Factor Concentration in Commercial Product by SECâ€HPLC

The commercial product, Mouse Nerve Growth Factor (NGF) for Injectionï¼Nobex®, is consisted of NGF and human albumin. These two components are both proteins and make it difficult to determine the concentration of NGF by conventional methods. In order to establish a new efficiency quality con... (18 Apr., 2011)

Cloning of an Ascorbate Peroxidase Gene from Puccinellia tenuiflora and its Expression Analysis

The full-length gene of an ascorbate peroxidase (PutAPx) was isolated from Puccinellia tenuiflora Ohwi cDNA library. The gene is 1 125 bp in length and it has an ORF of 876 bp, which encoded a protein of 291 amino-acid with an estimated molecular weight 32 kD and an isoelectric point of 7.71. Bla... (25 Feb., 2011)

The Development of a Multiplex Reverse Transcription Polymerase Chain Reaction for Detection of Porcine Reproductive and Respiratory

A multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) was developed for the detection of porcine reproductive and respiratory syndrome virus (PRRSV). A set of three pairs of primer were designed based on the sequence of high conservative region of PRRSV. The diagnostic ac... (24 Dec., 2010)

Differential Expression of P19, Cytochrome Oxidase and ALY- Family Genes in Resistant and Susceptible Tomato Cultivars (Solanum lycopersicum) Inoculated with Tomato Bushy Stunt Virus RETRACTED

Tomato cultivars (TY20, TY70/84 and TY70/70) were mechanically inoculated with purified virus preparations of Tomato bushy stunt virus (TBSV). Inoculated plant tissues were collected after one day, one week and two weeks post-inoculation, respectively. The P19 gene was amplified using specifi... (01 Jan., 1970)

Heterologous Expression and Purification of Cry1Ac22 Toxin from Bacillus thuringiensis W015-1

A cry1Ac22 gene was amplified by PCR from Bacillus thuringiensis strain W015-1 isolated from diapausing larvae of silkworm ( Bombyx mori ). The full-length gene was ligated into the prokaryotic expression vector pQE30 to construct the recombinant plasmid pQE30-Cry1Ac22 . The pQE30-Cry1Ac2... (27 Dec., 2010)

Differentiation of Gene Richness on Duplicated Chromosomes and Survey of Genes Captured by ESTs in Poplar Genome

In higher plant genomes, most sequences are unexpression sequence, the proportion of a genome that encodes for genes may be very small. Understanding gene distribution in the genome is a very important aspect for invastigating the genome structure. Funded by the U.S. Department of Energy, a clona... (29 Dec., 2010)

Cloning and Expression Analysis of Î²-ketoacyl-ACP Reductase, Î²-hydroxyacyl-ACP Dehydrase and Enoyl-ACP Reductase from Arachis hypogaea L.

Fatty acid biosynthesis is catalysed in most bacteria and plants by a group of highly conserved proteins known as the Type II fatty acid synthase (FAS) system. In this study, genes for β-ketoacyl-ACP reductase (KR), β-hydroxyacyl-ACP dehydrase (HD), and enoyl-ACP reductase (ENR) of Type... (28 Sep., 2010)

Fuel Loading and the Potential for Carbon Emissions from Fire Following Two Shelterwood Harvest Treatments in Southern Ohio

The purpose of this paper is to report the fuel load following shelterwood harvest of two intensities (70% residual stocking and 50% residual stocking), the total carbon content contained in these fuels, and the potential carbon emissions from fire. And the fuel load includes forest litter, woo... (27 Sep., 2010)

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