Wei Shi¹ , Takano Tetsuo² , Shenkui Liu¹

1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education,; Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin Hexing Road, 150040;
2. Asian Natural Environmental Science Center(ANESC), University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 188-0002, Japan
Author    Correspondence author
Molecular Soil Biology, 2012, Vol. 3, No. 2   doi: 10.5376/msb.2011.03.0002
Received: 29 Jun., 2011    Accepted: 01 Aug., 2011    Published: 09 Aug., 2011

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Shi et al., 2011, solation and characterization of an alkaliphilic and halotolerant Nesterenkonia.sp from an extreme soda saline-alkali soil in the northeastern of China, Molecular Soil Biology, doi:10.5376/msb.2011.03.0002

A strain of alkaliphilic and halotolerant, rod-shaped bacterium, designated strain SA-3, was solated from an Extreme soda saline-alkali soil in Heilongjiang, northeastern China. Strain SA-3 was Gram-positive, catalase-positive, and oxidase-negative and formed Orange colonies, and starch is not hydrolyzed, optimal growth conditions were pH 9.0 and 0.5 mol/L NaCl. Strain SA-3 has strong resistance to Na₂CO₃, hight pH value, and NaCl. 16S rRNA a gene sequence alignment analysis show that the strain formed a separated line within a clade containing the genus Nesterenkonia in the phylum Actinobacteria and was related to the species Nesterenkonia lutea YIM 70081^T (sequence similarity 99.2%), Nesterenkonia sandarakina YIM 70009^T (99.2%), Nesterenkonia jeotgali JG-241^T (99.1%), Nesterenkonia halotolerans YIM 70084^T (99.0%), Nesterenkonia aethiopica DSM 17733^T (97.5%). This similarity, suggest that this salt-tolerant alkaliphilic bacterium belonged to genus Nesterenkonia. This strain is a great potential microbial resources for development.

Nesterenkonia; 16S rDNA sequence