Jiangpo Chen¹ , Testuo Takano² , Shenkui Liu¹ , Yuanyuan Bu¹

1 Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040, China
2 Asian Natural Environmental Science Center (ANESC), The University of Tokyo, Tokyo 188-0002, Japan
Author    Correspondence author
Molecular Soil Biology, 2016, Vol. 7, No. 5   doi: 10.5376/msb.2016.07.0005
Received: 05 May, 2016    Accepted: 01 Jun., 2016    Published: 07 Jun., 2016

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Jiangpo Chen, Testuo Takano, Shenkui Liu, and Yuanyuan Bu, 2016, Identification and Characterization of a Cation2+/H+ Antiporter AtCAX4 Gene from Arabidopsis thaliana, Molecular Soil Biology, 7(5): 1-5 (doi: 10.5376/msb.2016.07.0005)

AtCAX4 is a member of the Ca²⁺/H⁺ exchangers (CAX) family was cloned from cDNA library of Arabidopsis thaliana. AtCAX4 was expressed in all Arabidopsis plant organs, with the highest level in root under normal conditions by quantitative real-time PCR analysis. The expression of AtCAX4 gene is response to Na⁺, Ca²⁺ and Ba²⁺ in both the leaves and roots of Arabidopsis. In addition, the pYES2-AtCAX4 transformants confers tolerance to Na⁺, Ca²⁺ and Ba²⁺ ions in yeast. These results suggest that AtCAX4 play important role in increasing the Na⁺, Ca²⁺ or Ba²⁺ tolerance of yeast.

AtCAX4; Gene expression; Tolerance; Yeast