S. Sankar¹ , B. Rohini¹ , K. Chairman² , S. Ramesh³ , S. Jayalakshmi³

1. CAS in Marine Biology, Annamalai University, Parangipettai-608502, Tamil Nadu, India
2. Dept. of Zoology, Sri Paramakalyani College, Alwarkurichi, Tamilnadu, India- 627 412
3. Dept. of Microbiology, Sri Paramakalyani College, Alwarkurichi, Tamilnadu, India- 627 412
Author    Correspondence author
Molecular Soil Biology, 2013, Vol. 4, No. 4   
Received: 25 Mar., 2013    Accepted: 02 Apr., 2013    Published: 02 Apr., 2013

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Enzymes are biocatalysts. They are widely used in many chemical reactions. Many enzymes such as lipases, amylases, proteases are of economic importance. Plants, animals and microbes produce these enzymes in a cost effective manner which is the need of the hour. The present study was carried out with the aim of microbial lipase production using Pseudomonas aeruginosa, isolated from Vellar river. Parameters such as inoculum load, pH, incubation time, temperature, substrate concentration variations and their influence on the lipase production was recorded. The present investigation reports that the optimum conditions for lipase production by the Pseudomonas aeruginosa as recorded was at pH 6, trybutyrin 2 mL, incubation time 96 hr, temperature 40℃. Oleic acid content, free fatty acid concentration, lipase activity of the enzyme were also carried out and the crude enzyme was purified by dialysis, ammonium sulphate precipitation. The molecular weight of the purified enzyme was found to be 37-77 KDa. This study thus suggests that the enzyme produced from the optimized media can be further technologically improved and applied for industrial application.

Pseudomonas aeruginosa; Extracellular lipase; Optimization; Purification; Tributyrin