Zhangbao Huang , Hui Ren , Long Dong , Zhaoyan Cai , Weikuan Fang , Xiaomei Wang , Weiqiang Su , Yeqiang Liu , Wenwu Qiu

South Asian Tropical Fruit Tree Scientific Observation Station (Nanning), Ministry of Agriculture and Rural Affairs, Horticultural Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, China
Author    Correspondence author
Bioscience Methods, 2023, Vol. 14, No. 1   doi: 10.5376/bm.2023.14.0001
Received: 14 Feb., 2022    Accepted: 20 Oct., 2022    Published: 17 Mar., 2023

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Huang Z.B., Ren H., Dong L., Cai Z.Y., Fang W.K., Wang X.M., Su W.Q., Liu Y.Q., and Qiu W.W., 2023, A modified method of total RNA isolation for mango leaf tissues, Bioscience Method, 14(1): 1-8 (doi: 10.5376/bm.2023.14.0001)

Complex metabolic components in mango leaves lead to high difficulty in RNA extraction. In order to improve the quality of total RNA extraction, on the basis of the existing methods reported in the literature, an RNA extraction method combining acetone washing liquid nitrogen abrasive material with 0.3 mol/L lithium chloride (LiCl) solution and 2 times the volume of anhydrous ethanol as the second precipitator was developed. The experimental results showed that the improved method significantly reduced the amount of impurity precipitation in the RNA extraction process. The electrophoretic bands of total RNA extracted were complete. The absorbance ratio of OD_260/280 was about 1.9, and the average was 329.8 g/g FW±11.2 g/g FW. Further validation experiments of RNA reverse transcription and the polymorphic amplification of double-stranded cDNA related sequences (RAP-PCR) obtained clear polymorphic bands, showing that the total RNA from mango leaves had high quality and was suitable for molecular biology experiments.

Mango; RNA abstraction method; Polysaccharides; Polyphenols