Effects of Salt-alkaline Stress on Chlorophyll Fluorescence Characteristics in Leaves of Chieh-qua Seedlings
1 Shanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China
2 Shanghai Shangshi Modern Agricultural Development Co., Ltd, Shanghai, 202183, China
Bioscience Methods, 2023, Vol. 14, No. 5 doi: 10.5376/bm.2023.14.0005
Received: 10 Oct., 2023 Accepted: 17 Oct., 2023 Published: 27 Oct., 2023
© 2023 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Lu P.L., Du X., Wang Y., Zhang H.M., Tian S.B., Wang N., and Liu N., 2023, Effects of salt-alkaline stress on chlorophyll fluorescence characteristics in leaves of Chieh-qua seedlings, Bioscience Method, 14(5): 1-8 (doi: 10.5376/bm.2023.14.0005)
To explore the effect of salt-alkaline stress on the growth and chlorophyll fluorescence characteristics of Chieh-qua seedlings, the Chieh-qua inbred line "C-39" was used as the experimental material, nutrient solution culture was used for the experiment, and different concentrations of alkaline salts NaHCO3 (0, 25, 50, 75, 100, 125 mmol/L) were used for gradient treatment. The results showed that the biomass, plant height and stem thickness of the seedlings showed a decreasing trend with the increase of NaHCO3 concentration, which means that the salt stress seriously inhibited the growth of the seedlings. High concentration of NaHCO3 significantly inhibited the activity of PSII, when the concentration of NaHCO3 reached 75 mmol/L, Fv/Fm and PIabs were significantly lower than CK, while low concentration of NaHCO3 treatment had no significant effect on the activity of PSII. Analysis of JIP-test showed that the relative variable fluorescence VL, VK, VJ and VI at 0.15, 0.3, 2 and 30 ms on the OJIP curve were significantly higher than CK when the concentration of NaHCO3 reached 75 mmol/L, but there were no significant differences between low concentrations. It was explained that high concentration of NaHCO3 treatment inhibited the electron transport of PSII. The increase of VJ was greater than that of VK, indicating that the damage caused by NaHCO3 stress on the donor side was greater than that in the receptor side of the Chieh-qua seedlings. Moreover, the optical energy absorption and distribution in leaves of Chieh-qua seedlings were significantly influenced by high NaHCO3 concentrations treatment. The concentration of reaction centers RC/CSm (Expressed as per unit leaf area) and the proportion of absorbed light energy used for electron transport were decreased. In summary, NaHCO3 treatment inhibited the growth of Chieh-qua seedlings and high-concentration NaHCO3 treatment severely inhibited the Chieh-qua seedlings’ photosynthesis. This study provides a theoretical basis for the creation of salt-tolerant Chieh-qua germplasm resources by studying the effects of different concentrations of alkaline salt stress on the growth and photosynthetic performance of seedlings.
Chieh-qua; Salt-alkaline stress; Chlorophyll fluorescence; PSII