Mengmeng Yin , Fan Luo , Xiaoli Zhou , Changhe Wei , Qian Zhu , Huirong Dong , Lijuan Chen , Dong Sun Lee

1 State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, Yunnan, China
2 The Key Laboratory for Crop Production and Smart Agriculture of Yunnan Province, Yunnan Agricultural University, Kunming 650201, Yunnan, China
3 Rice Research Institute, Yunnan Agricultural University, Kunming, 650201, Yunnan, China
4 College of Agricultural Science, Xichang University, Liangshan, 615013, Sichuan, China
5 Panxi Crops Research and Utilization Key Laboratory of Sichuan Province, Xichang University, Liangshan, 615013, Sichuan, China
Author    Correspondence author
Bioscience Methods, 2024, Vol. 15, No. 4   doi: 10.5376/bm.2024.15.0019
Received: 05 Jul., 2024    Accepted: 17 Aug., 2024    Published: 29 Aug., 2024

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Yin M.M., Luo F., Zhou X.L., Wei C.H., Zhu Q., Dong H.R., Chen L.J., and Lee S.D., 2024, Unraveling the functional role of R2R3-MYB genes in black rice anthocyanin pathway: a CRISPR/Cas9 and overexpression study, Bioscience Methods, 15(4): 184-195 (doi: 10.5376/bm.2024.15.0019)

R2R3-OsMYBs play a pivotal role in the regulation of anthocyanin biosynthesis, which is responsible for the black pigmentation observed in rice. This paper is aimed to overview the functional mechanisms of these genes through the application of CRISPR/Cas9 gene editing and overexpression techniques. Recent studies have demonstrated the effectiveness of CRISPR/Cas9 in targeting key genes within the anthocyanin biosynthesis pathway, including OsF3'H, OsDFR, and OsLDOX, leading to significant alterations in anthocyanin content and seed color in rice. Moreover, the overexpression of R2R3-MYB genes, such as OsC1, has been demonstrated to increase anthocyanin production and enhance oxidative stress tolerance in rice. Comparative studies in other plant species, including tomato and Nitraria sibirica, have further illuminated the diverse regulatory roles of R2R3-MYB TFs in anthocyanin biosynthesis. This review summarizes current findings to provide a comprehensive understanding of the genetic and molecular basis of anthocyanin regulation in black rice, offering insights into potential applications for crop improvement and functional food development.

Black rice; R2R3-MYB; Anthocyanin biosynthesis; CRISPR/Cas9; Overexpression