Yanxin Ma , Shuo Yang , Shuping Lang

Jiaxing Academy of Agricultural Sciences, Jiaxing, 314016, Zhejiang, China
Author    Correspondence author
Bioscience Methods, 2024, Vol. 15, No. 6   
Received: 24 Sep., 2024    Accepted: 05 Nov., 2024    Published: 24 Nov., 2024

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

This study discusses the application of CRISPR/Cas9 technology in wheat genetic improvement, highlighting its potential in overcoming traditional breeding limitations. The study covers the working principles of CRISPR/Cas9, its advantages in precise genome editing, and its utility in enhancing traits such as disease resistance, stress tolerance, yield, and nutritional content in wheat. Case studies illustrate successful implementations where susceptibility genes were edited to bolster disease resistance, and specific genes were targeted to improve stress tolerance and grain quality. Despite its potential, challenges such as low editing efficiency, off-target effects, and regulatory hurdles remain. Nonetheless, the integration of CRISPR/Cas9 with other methods shows promise for future wheat breeding, aiming to ensure food security amidst growing global demands.

CRISPR/Cas9; Wheat genetic improvement; Disease resistance; Stress tolerance; Genome editing