Fei Zhao , Jinni Wu

Aquatic Biology Research Center, Cuixi Academy of Biotechnology, Zhuji, 311800, Zhejiang, China
Author    Correspondence author
Bioscience Methods, 2025, Vol. 16, No. 3   
Received: 17 Apr., 2025    Accepted: 28 May, 2025    Published: 20 Jun., 2025

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

This study analyzed the hazards and immune response mechanisms of common diseases of snakehead fish in recent years (such as nocardia, Aeromonas hydrophila, viral hemorrhagic septicemia, etc.), and summarized the mining methods and functional research progress of key genes for disease resistance of snakehead fish, including screening of immune genes such as IL-17 and TRAF through whole genome scanning and transcriptomics. At the same time, the application status and advantages of CRISPR/Cas9 gene editing technology in aquaculture were discussed, such as efficient site-directed mutagenesis and introduction of exogenous antimicrobial peptide genes to enhance fish disease resistance. Through case analysis of the successful experience of disease-resistant gene editing in related fish (such as Atlantic salmon and catfish), this study prospected the potential path and results of disease-resistant gene editing breeding of snakehead fish, and discussed its ecological and ethical impacts (such as off-target effects, food safety and public acceptance, etc.), which is of great significance to improving aquaculture production and disease prevention and control, and also provides the latest theoretical basis and practical reference for disease-resistant breeding of snakehead fish.

Snakehead; Disease resistance gene; Immune mechanism; CRISPR/Cas9; Gene editing breeding