Zhigang Fu

Jiashan County Fengchan Grain & Oil Professional Cooperative, Jiashan, 314110, Zhejiang, China
Author    Correspondence author
Bioscience Methods, 2025, Vol. 16, No. 4   
Received: 03 Jun., 2025    Accepted: 14 Jul., 2025    Published: 02 Aug., 2025

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

In the context of the current green transformation of agriculture and sustainable ecological development, improving the nitrogen use efficiency of rice has become an important issue to ensure food security and reduce environmental pollution. This study systematically explored the key physiological mechanisms of rice in the process of nitrogen absorption, transport and metabolism, focusing on the expression and regulatory role of key genes such as OsNRT1.1B, OsAMT1.2, and OsGS1;1, as well as the functions of transcription factors such as NLP, DOF, and MYB in the regulation of nitrogen metabolism. Combined with the development trend of green agriculture in recent years, this study further evaluated the practical effects of the "one base and one topdressing" fertilization mode of controlled-release fertilizers and the integrated management with green control technology, and analyzed the synergistic effect of high-efficiency varieties and green fertilization modes through a typical case of the demonstration field in Dahao Village, Jiashan. The study showed that indica-japonica hybrid rice and excellent late japonica rice varieties have a strong responsiveness to nitrogen supply, and can significantly improve nitrogen use efficiency and yield stability under reasonable cultivation management and precision fertilization. This study not only provides theoretical support and practical path for reducing nitrogen fertilizer and increasing its efficiency, but also provides a reference for the construction of regional rice ecological planting system.

Rice; Nitrogen use efficiency; Green fertilization; Physiological mechanism; Molecular regulation