Xiaoqing Tang

Hainan Institute of Biotechnology, Haikou, 570206, Hainan, China
Author    Correspondence author
Bioscience Methods, 2025, Vol. 16, No. 6   doi: 10.5376/bm.2025.16.0030
Received: 15 Oct., 2025    Accepted: 26 Nov., 2025    Published: 19 Dec., 2025

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Tang X.Q., 2025, Case study on the use of Assisted reproductive techniques in improving water buffalo fertility, Bioscience Methods, 16(6): 308-316 (doi: 10.5376/bm.2025.16.0030)

 

Water buffaloes play a significant role in livestock production in many regions of Asia, undertaking multiple functions such as dairy production, meat processing, and draft use. However, its reproductive efficiency is relatively low, such as long postnatal intervals and difficulty in identifying estrus, which have long restricted the improvement of production performance and the progress of germplasm improvement. This study systematically reviewed the reproductive biological characteristics of water buffaloes, the limitations of natural reproduction, and analyzed the mechanism of ARTs in improving reproductive performance, including enhancing conception rates, synchronous estrus, and accelerating genetic progression. Through the case analysis of the Indian Buffalo Breeding Center, the artificial insemination promotion project in the Philippines, and the OPU-ET experimental platform in southern China, this study evaluated the effectiveness, advantages, and technical and management challenges faced by ARTs in practical applications. The research results show that although ARTs can significantly improve the reproductive efficiency of water buffaloes and promote the rapid spread of superior genes, its large-scale promotion still relies on cost reduction, farmer training and the construction of a good supporting system. This study aims to reveal the mechanisms by which these techniques improve the reproductive performance of water buffaloes, shorten their reproductive cycles, and promote the expansion of superior populations, and to provide theoretical support and practical references for establishing a scalable and sustainable water buffalo breeding technology system.

Water buffalo; Assisted reproductive technology; Reproductive capacity; Artificial insemination; Embryo transfer