Jilai Lu¹ , Zhiyong Wang¹ , Xiangyu Long² , Yongjun Fang² , Mingxu Zhou³ , Jianghua Yang² , Yunxia Qin²

1 College of Tropical Crop, Hainan University, Haikou, 570228, China
2 Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China
3 College of Tropical Crop Science, Yunnan Agricultural, PuEr, 665000, China
Author    Correspondence author
Computational Molecular Biology, 2022, Vol. 12, No. 2   doi: 10.5376/cmb.2022.12.0002
Received: 09 Feb., 2022    Accepted: 20 Feb., 2022    Published: 19 Mar., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Lu J.L., Wang Z.Y., Long X.Y., Fang Y.J., Zhou M.X., Yang J.H., and Qin Y.X., 2022, Characterization and expression profiles of galaetinol synthase and raffinose synthase in rubber tree, Computational Molecular Biology, 12(2): 1-7 (doi: 10.5376/cmb.2022.12.0002)

Galactinol synthase (GolS) and Raffinose synthase (RS) are key enzymes for the synthesis of Raffinose family oligosaccharides (RFOs) which are widely involved in the growth development and resistances to stresses. To explore the function of HbGolSs and HbRSs during leaf development and tapping period in Hevea brasiliensis, in this study, based on the established library data platform of rubber tree genome, we isolated and identified the family members of HbGolSs and HbRSs, then we analyzed their expression profiles with emphasis through on-line molecular biological tools and PRISM software as well. The results demonstrated that HbGolS1 and HbRS1 were prime member in their separate family, HbGOlS1 was highly expressed in latex and leaf, and with leaf development, its level gradually increased till the ultimate in mature leave, especially, it was dramatically up-regulated by tapping but markedly inhibited by Ethrel treatment; Different from it, HbRS1 was little influenced by tapping or Ethrel treatment although it was specially expressed in latex. Together, this study demonstrated the expression characteristics of HbGolS1 and HbRS1 with leaf development and tapping, which has laid a foundation for further exploring the metabolism of RFOs and their roles in rubber trees.

Adversity stress; Galaetinol synthase; Raffinose synthase