Kaili Yu¹ , Zhichang Zhao² , Aiping Gao² , Ruixiong Luo² , Jianfeng Huang² , Mengyun Zhang² , Maofu Li¹

1 College of Horticulture, Hainan University, Haikou, 570228, China
2 National Cultivar Improvement Center of Tropical Fruit Tree, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Tropical Crops Genetic Resources Institute Chinese Academy of Tropical Agricultural Sciences, Haikou, 571701, China
Author    Correspondence author
Computational Molecular Biology, 2022, Vol. 12, No. 4   doi: 10.5376/cmb.2022.12.0004
Received: 14 Jul., 2022    Accepted: 15 Aug., 2022    Published: 28 Sep., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Yu K.L., Zhao Z.C, Gao A.P., Luo R.X., Huang J.F., Zhang M.Y., and Li M.F., 2022, Cloning and expression analysis of phytoene desaturase (PDS) gene from mango (Mangifera indica L.), Computational Molecular Biology, 12(4): 1-8 (doi: 10.5376/cmb.2022.12.0004)

Phytoene desaturase (PDS) affects the synthesis of carotenoids. It is a key gene in the carotenoids biosynthesis pathway. In order to study the function of PDS gene from mango fruits, the phytoene dehydrogenase (PDS) gene of ‘Guifei’ mango fruit was obtained with RACE methods. The full-length cDNA sequence of the gene is 1 820 bp, open reading frame is 1 650 bp, encoding 549 amino acids, the molecular weight is 61.34 KD, and the isoelectric point is 6.78. It was cluster analysis found that the mango PDS protein had a close relationship with grapefruit, cantaloupe, and papaya. Its amino acid composition is mainly alanine (ALa), leucine (Leu), valine (Val), and so on. Expression of PDS gene in different varieties by PCR showed: the high expression of the red ‘Guifei’ varieties and the expression of green mango varieties with low volume. Prediction of the domain, tertiary structure, and interaction protein of its protein, found to contain phytoene-desat, PLNO2487 superfamily, and other domains. The use of the STRING database found PDS interacted with proteins such as PSY, ZDS, and CRTISO. This research will be valuable for understanding the molecular mechanism of gene regulation in carotenoid biosynthesis and can be served as the basis for the metabolic engineering of mango.

Mango; PDS; Gene cloning