Wenzhong  Huang

Biomass Research Center, Hainan Institute of Tropical Agricultural Resouces, Sanya, 572025, Hainan, China
Author    Correspondence author
Computational Molecular Biology, 2025, Vol. 15, No. 1   
Received: 03 Jan., 2025    Accepted: 12 Feb., 2025    Published: 24 Feb., 2025

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

In recent years, enzymatic and ultrasonic-assisted extraction techniques, as green and efficient extraction strategies, have gradually been applied in the extraction and development of natural products such as Cordyceps polysaccharides. This study systematically explored the application of enzymatic and ultrasonic-assisted extraction techniques in the extraction of Cordyceps polysaccharides, analyzed the synergistic effects of the two techniques, and evaluated their impacts on extraction efficiency, biological activity and environmental benefits. The research results show that enzymatic extraction enhances the efficiency of polysaccharide release by selectively decomposes the cell wall, while ultrasonic-assisted extraction significantly improves the efficiency of solvent penetration and diffusion through cavitation effects. The combined application of the two technologies has further enhanced the extraction rate, polysaccharide yield and purity, while significantly reducing energy consumption and solvent consumption. The Cordyceps polysaccharides obtained through co-extraction exhibit stronger antioxidant capacity and immunomodulatory activity. This study provides a new idea for the green extraction of Cordyceps polysaccharides, systematically evaluates the application advantages and synergistic effects of enzymatic and ultrasonic-assisted technologies, promotes the green, precise and efficient development of Cordyceps polysaccharide extraction technology, and offers a reference for the sustainable development and industrialization of natural products.

Cordyceps polysaccharides; Enzymatic extraction; Ultrasound-assisted; Green extraction technology; Synergistic effect