Research Article
Sustainable Biomethane Generation and Process Optimization from Human Excreta and Household Food Waste for Renewable Vehicle Fuel Applications 
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Journal of Energy Bioscience, 2026, Vol. 17, No. 1
Received: 07 Nov., 2025 Accepted: 29 Dec., 2025 Published: 06 Feb., 2026
Rapid urbanization and waste generation have intensified the demand for sustainable energy and waste management solutions. This study investigates biomethane production through the co-digestion of household food waste (FW) and human excreta (HE), targeting renewable vehicle fuel applications. Laboratory-scale experiments were designed using the Taguchi method to optimize key parameters, including substrate mixing ratio, pH, retention time, and inoculum concentration. Results showed that co-digestion enhanced biomethane yield significantly, achieving 410 mL CH₄/g VS compared to 275 mL/g VS (FW alone) and 180 mL/g VS (HE alone). Process stability was improved by maintaining optimal volatile fatty acid to alkalinity ratios, reducing the risk of digester failure. Upgrading trials via membrane separation produced methane concentrations above 95%, meeting vehicle fuel standards. Techno-economic assessments indicated favorable cost-benefit performance, particularly when digestate utilization and carbon credits were included. Furthermore, pathogen inactivation protocols ensured safe digestate reuse, enhancing environmental and public health outcomes. Compared to existing literature, this study demonstrates superior methane yield and improved scalability prospects under real-world waste variability. The findings highlight the feasibility of integrating FW and HE co-digestion into urban renewable energy infrastructures, offering a sustainable pathway for circular economy and climate change mitigation.
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