Xu Liu , Zeyu Jin , Haoda Liu , Yuanyuan Bu

Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, China
Author    Correspondence author
Molecular Soil Biology, 2026, Vol. 17, No. 1   
Received: 17 Dec., 2025    Accepted: 20 Jan., 2026    Published: 06 Feb., 2026

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

To investigate the effects of EDTA-Ca and EDTA-Fe on microalgal biomass accumulation, this study used the salt-tolerant strain SA-2, isolated and screened from soda-alkali soil in Anda, Heilongjiang Province. SA-2 was treated with different concentrations of EDTA-Ca and EDTA-Fe to explore the effects of metal chelator–mediated micronutrient regulation on its growth and physiological–biochemical characteristics.The results showed that when the concentration of EDTA-Ca was 0.3 mg/L or that of EDTA-Fe was 0.2 mg/L, both the cell density and dry weight of SA-2 reached their highest levels, indicating optimal growth. At these concentrations, the lipid, chlorophyll, carbohydrate, and protein contents of the microalgae also reached their maximum values.This study identifies the optimal concentrations of EDTA-Ca and EDTA-Fe that promote the growth and biomass accumulation of SA-2, providing experimental evidence and theoretical guidance for efficient microalgae cultivation and biomass resource utilization.

Microalgae; EDTA-Ca; EDTA-Fe; Biomass content