Case study
Soil Microbial Community Changes under Continuous Cucumber Cropping in Greenhouse Systems 
2 Zhejiang Agronomist College, Hangzhou, 310021, Zhejiang, China
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Correspondence author
Molecular Soil Biology, 2026, Vol. 17, No. 1
Received: 03 Jan., 2026 Accepted: 05 Feb., 2026 Published: 25 Feb., 2026
Continuous cropping of greenhouse cucumbers is widely practiced in intensive vegetable production, but long-term monoculture often leads to soil degradation and continuous cropping obstacles. Soil microbial communities play a key role in maintaining soil fertility, nutrient cycling, and plant health. This study explores the changes in soil microbial community structure under greenhouse cucumber continuous cropping conditions. By combining soil physicochemical analysis with high-throughput sequencing techniques, the diversity and composition of soil microorganisms were examined under different continuous cropping durations. The results show that prolonged continuous cropping significantly alters microbial community structure, reduces microbial diversity, and increases the abundance of certain pathogenic microorganisms. Environmental factors such as soil pH, organic matter, and root exudates were identified as important drivers of these changes. The study highlights the importance of microbial regulation strategies, including bio-organic fertilizers and crop rotation, to improve soil health and promote sustainable greenhouse cucumber production.
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. Lingli Shen
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