Zhanhui Jia^1,2 , Xiaodong Jia^1,2 , Mengyang Xu^1,2 , Zhenghai Mo^1,2 , Xufeng Yang^1,2 , Min Zhai^1,2 , Jiping Xuan^1,2 , Jiyu Zhang^1,2 , Gang Wang^1,2 , Tao Wang^1,2 , Zhongren Guo^1,2

1 Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China
2 The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, 210014, China
Author    Correspondence author
Bioscience Methods, 2022, Vol. 13, No. 1   doi: 10.5376/bm.2022.13.0001
Received: 25 Nov., 2021    Accepted: 07 Jan., 2022    Published: 18 Jan., 2022

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Jia Z.H., Jia X.D., Xu M.Y., Mo Z.H., Yang X.F., Zhai M., Xuan J.P., Zhang J.Y., Wang G., Wang T., and Guo Z.R., 2022, Cloning and bioinformatics analysis of genes related to key enzymes in oil metabolism of Carya illinoensis (Wangech.) K. Koch, Bioscience Method, 13(1): 1-13 (doi: 10.5376/bm.2022.13.0001)

Taking mixed sample of 115 d and 135 d Carya illinoinensis ‘Pawnee’ as experimental materials, after RT-PCR amplification, cloning and sequencing, the gene sequences of CiSAD gene, CiFAD2 gene and CiGPAT gene related to the key enzymes of oil metabolism in Carya illinoinensis were obtained, and bioinformatics analysis was carried out. The length of CiSAD gene was 1 240 bp, containing a complete open reading frame (ORF) of 1 194 bp, encoding 397 amino acids. CiFAD2 was 1 329 bp, containing an ORF of 1 155 bp, encoding 384 amino acids. CiGPAT was 1 671 bp, containing an ORF of 1 626 bp, encoding 541 amino acids. All proteins encoded by the cloned genes have corresponding domains, indicating that the proteins encoded by these genes should have corresponding functions in organisms.

Carya illinoensis (Wangech.) K. Koch; Oil metabolism; Gene cloning; Bioinformatics analysis