Hong Yang^1* , Jin Chen^2* , Chunlong Zhang¹ , Khadija Tehseen Arshad¹ , Qiuping Li¹ , Tuo Wang¹ , Jiancheng Wen¹ , Dandan Li²

1 Rice Farming Research Institute, Yunnan Agricultural University, Kunming, 650201, P.R. China
2 College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, P.R. China
Author    Correspondence author
Bioscience Methods, 2022, Vol. 13, No. 2   doi: 10.5376/bm.2022.13.0002
Received: 13 Jan., 2022    Accepted: 20 Jan., 2022    Published: 21 Feb., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Yang H., Chen J., Zhang C.L., Arshad K.T., Li Q.P., Wang T., Wen J.C., and Li D.D., 2022, Construction of rice OsSUTs gene knockout lines using CRISPR/Cas9 system, Bioscience Method, 13(2): 1-8 (doi: 10.5376/bm.2022.13.0002)

The efficiency of sucrose transfer directly affects starch accumulation in rice grains and rice yield. There are five sucrose transporters reported in rice, the relationships of them are complex, and the interaction mechanisms of them are still unclear. In this study, based on the sequence information of the five OsSUTs genes in rice, target sites were designed respectively, the corresponding CRISPR/Cas9 vector was successfully constructed, and five positive knock-out seedlings of OsSUTs genes were obtained through transgenic technology. It provides a good material for exploring the molecular function and regulatory relationship of OsSUTs gene.

Rice (Oryza sativa L.); OsSUT genes; Gene knockout; CRISPR/Cas9