Yueyuan Zhang , Mengxuan Hu , Bao Di , Duanfen Chen

College of horticulture, Hebei Agricultural University, Baoding, 071001, China
Author    Correspondence author
Bioscience Methods, 2023, Vol. 14, No. 3   doi: 10.5376/bm.2023.14.0003
Received: 11 Apr., 2022    Accepted: 05 Apr., 2023    Published: 06 May, 2023

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Zhang Y.Y., Hu M.X., Di B., and Chen D.F., 2023, Establishment of tissue culture and rapid propagation system on Mandevilla sanderi, Bioscience Method, 14(3): 1-9 (doi: 10.5376/bm.2023.14.0003)

Aimed at the problems of low reproductive coefficient and long seeding period for Mandevilla sanderi ‘Sunparasol’, the rapid propagation system was studied in this article. Research results showed as follows: The disinfectant and disinfection time for young stem segments of M.sanderi is 0.1% HgCl₂ for 3 min; When the culture medium is MS+6-BA 4 mg/L+NAA 0.1 mg/L, the germination rate is as high as 93.33%; In the subculture stage, the number of multiplication and growth is strong in the medium of MS+6-BA 2 mg/L+NAA 0.1 mg/L, and the multiplication coefficient is 4.31; Sucrose concentration of 40 g/L can promote the growth of fine tissue culture seedlings; the optimum growth condition of test tube seedlings is the best when 1~2 mg/L of CCC is added; The suitable rooting medium is 1/2MS+NAA 0.1 mg/L, the rooting rate is 83.33%, and the average rooting number is 4.3. The above-mentioned establishment of tissue culture rapid propagation system has important guiding significance for the large-scale production of M.sanderi.

Mandevilla sanderi; Tissue culture; Rapid propagation system