Biao Lai¹ , Chunfan Chen¹ , Zehui Tang¹ , Jing Xiao² , Qi Wang¹ , Fabo Chen¹ , Lina Du¹

1 School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Fuling, 408100, P.R. China
2 Fujian EcoTech Center for State Farm and South Subtropical Crops, Fuzhou, 350003, P.R. China
Author    Correspondence author
Computational Molecular Biology, 2022, Vol. 12, No. 1   doi: 10.5376/cmb.2022.12.0001
Received: 22 Dec., 2021    Accepted: 14 Jan., 2022    Published: 28 Feb., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Lai B., Chen C.F., Tang Z.H., Xiao J., Wang Q., Chen F.B., and Du L.N., 2022, Genome-wide identification and expression analyses of PAL genes in different color radish, Computational Molecular Biology, 12(1): 1-9 (doi: 10.5376/cmb.2022.12.0001)

Phenylalanine ammonia-lyase (PAL) is the first step key enzyme of the flavonoid biosynthesis pathway which played an essential role in plant anthocyanin accumulation. Five PAL family gene members were identified in the radish (Raphanus sativus L.) genome name RsPAL1~5. Specific primers were designed to amplify the open reading frame in red radish ‘Hongxin No.1’ and then sequenced. Real-time PCR was used to analysis the expression pattern of 5 RsPALs in four different tissues including leaf, petiole, taproot flesh and skin form five different color type radishes, including red skin and red flesh ‘Shaguan’ and ‘Hongxin No.1’, red skin and white flesh ‘Shaguan No.1’ and ‘Mantanghong’, white skin and white flesh ‘Chunbulao’. The results showed that the length of open read frame of RsPAL1~5 were 2 160, 2 166, 2 163, 2 124 and 2 109 bp encoding for 719, 721, 720, 707 and 702 amino acid residues respectively. Sequence alignment analysis showed that MIO motif (Ala-Gly-Ser) was conserved among the five RsPALs proteins. RsPAL1~4 were clustered with Arabidopsis AtPAL1 and AtPAL2, RsPAL5 was clustered with AtPAL4 in the phylogenetic tree. Real-time PCR results suggested that RsPAL4 was expressed only in the tissue accumulate anthocyanin, and the expression of RsPAL4 was significantly correlated with anthocyanin content. These results indicating that RsPAL4 may specifically involved in anthocyanin biosynthesis in radish. However, no obvious expression pattern of other RsPALs members was found in this study, suggesting that they may participate in other secondary metabolites biosynthesis of phenylpropane metabolism pathway. This study would provide scientific basis for further study on the function of radish PAL.

Carmine radish; PAL; Anthocyanin; Gene expression