Luo Lan^1,2 , Si Xiuyang³ , Sun Lei^1,2 , Gao Peng^1,2 , Li Yong Yong² , Wang Xuezheng^1,2

1 College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030, China
2 Key Laboratory of Biology and Genetic Improvement Horticultural Crops (Northeast Region), Ministry of Agriculture, Northeast Agricultural University, Harbin, 150030, China
3 College of Agriculture, Northeast Agricultural University, Harbin, 150030, China
Author    Correspondence author
Computational Molecular Biology, 2022, Vol. 12, No. 5   doi: 10.5376/cmb.2022.12.0005
Received: 22 Jul., 2022    Accepted: 25 Aug., 2022    Published: 30 Sep., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Luo L., Si X.Y., Sun L., Gao P., Li Y., and Wang X.Z., 2022, Identification and expression characteristic analysis of CML gene family of melon, Computational Molecular Biology, 12(5): 1-13 (doi: 10.5376/cmb.2022.12.0005)

Calmodulin-like (CML) is one of the important Ca²⁺ sensors, which plays an important role in plant growth and development and stress response. In this study, melon (Cucumismelo L.) genomic data was used to identify the melon CML protein family using bioinformatics methods, and its physical and chemical properties, location information, gene structure, phylogeny, and promoter were analyzed. The results showed that 60 CmCML protein genes were identified in the melon genomic data, containing 1~4 EF-hand domains, which were unevenly distributed on 12 chromosomes. By analyzing the evolutionary treewith Arabidopsis CMLs, CmCML could It is divided into 8 groups, and the number of various groups is different. It has certain similarities with other plant CML family genes in group classification. In the promoter analysis, abscisic acid, jasmonic acid, auxin, and gibberellin were identified. And drought, low temperature, mechanical damage and other signal response homeopathic elements. Real-time fluorescence quantitative results showed that gene expression of melon CML protein family was induced by the specialization of Fusarium oxysporum, these results predicted that the transcriptional regulation of melon CML protein family may participate in the resistance response of melon wilt, which is the gene family. The functional identification of each member laid a theoretical foundation.

Melon; CML; Bioinformatics; Expression analysis